Innovative nanomaterials for detection of PIK3CA DNA point mutations for breast cancer therapy

Beschreibung

NanoPredict (FFG-Nr.:FO999899038) targets the sensitive and selective detection of the three predominant PIK3CA point mutations (H1047R, E545K, and E542K), for the treatment of metastatic hormone receptor-positive, HER2-negative breast cancer. For this purpose, a point mutation-specific detection assay on multi-channel screen-printed gold sensors combined with isothermal recombinase polymerase amplification (RPA) was developed. With this assay, a limit of detection (LOD) of 224 copies/μL and a PIK3CA point mutations’ specificity of >20% could be reached. Based on these results, different amplification methods were compared to improve the sensitivity and specificity of PIK3CA mutation detection in cell-free DNA (cfDNA) obtained from plasma samples.
In addition to the assay development, the presentation addresses the research towards the realization of a POC-compatible, highly scalable chip platform. Results on screen-printable thermal elements, high-purity nanomaterial inks, nanozymes and printable surface modifications (DNA probes) will be presented.
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Zeitraum	12 Juni 2025
Ereignistitel	BioCHIP Berlin: International Forum on BioChips and Microfabriation
Veranstaltungstyp	Konferenz
Ort	Berlin, DeutschlandAuf Karte anzeigen