Microarray analysis of methane and methanol-degrading methylotrophic bacteria using phylogenetic (16S rRNA) and functional genes

  • Shrisuda Dhamwichukorn (Autor)
  • Levente Bodrossy (Autor)
  • F. Kulpa Charles (Autor)

Aktivität: Vortrag ohne Tagungsband / VorlesungPräsentation auf einer wissenschaftlichen Konferenz / Workshop


Methylotrophs have been of great interest because of their importance in bioremediation of environmental and industrial wastes and their potential commercial applications. In this study, oligonucleotide microarrays were employed for the analysis of methane and methanol degrading methylotrophs using both 16S rRNA genes and related functional genes (e.g., mxaF and pmoA) as probes. Two methane and methanol utilizing methylotrophs (i.e. Methylococcus capsulatus and Methylobacterium extroquens, respectively) were selected as model bacteria. The two bacteria were grown in minimal liquid media containing methane or methanol both separately and together. In addition, agar media such as TSA, R2A, and NA were used to grow the methanol-degrading bacterium. The oligonucleotide probe set was designed using the phylogenetic software ARB, based on databases of the available gene sequences, guided by phylogenetic trees. The designed probes were spotted onto microarray glass substrates and covalently immobilized using amino-aldehyde chemistry. These arrays were used to detect the genes of two bacteria in the various media and conditions. The results indicated that the two methylotrophs can co-exist in the same microcosm. Overall, the results demonstrate the feasibility of using microarrays for methane and methanol degrading methylotrophic detection and identification with those probes. The ability of using the functional genes as probes to detect the metabolic gene expression is being tested.
Zeitraum10 Aug. 200314 Aug. 2003
Ereignistitel2003 Annual Meeting - Industrial Microbiology and Biotechnology

Research Field

  • Nicht definiert