Surface Modification of Smart Sensors & Microfluidics for Life Sciences

Aktivität: Vortrag ohne Tagungsband / VorlesungPräsentation auf einer wissenschaftlichen Konferenz / Workshop


To serve the increasing demand for low-cost to manufacture, easy-to-use point-of-care (POC) diagnostic systems for healthcare and lifestyle, upscaling of the underlying biosensor system concepts using novel functionalization strategies for sample preparation or signal generation is crucial. The Molecular Diagnostics competence unit of the AIT Austrian Institute of Technology GmbH addresses this need by investigating key research issues for low-volume (picoliter/nanoliter) inkjet printing or “high volume” spotting techniques (microliter), and therefore, works on the development of printing processes and (bio)inks for reproducible batch production, e.g. for functional (bio)sensors, immuno- or enzymatic assays, as well as nucleic acid diagnostic devices. In this presentation, we will review results related to these aspects from three different research projects, namely the Greensense[1,2] (H2020, No 76100), ELSAH (H2020, No 825549)[3] and Hydrochip2 project (FFG._Nr. 883914)[4]. The Greensense project focused on the development of a paper based electrochemical sensor for the detection of drugs of abuse by a label-free electrochemical assay. Crucial for the assay was the development of a redox-active methylene blue-graphene oxide ink and its processing by spotting techniques for the reproduceable modification of amperometric sensors. The ELSAH project aims at the realization of a wearable, microneedle-based biosensor system for the continuous monitoring of glucose and lactate in the interstitial fluid for lifestyle diagnostics. Formulated bioinks comprise direct electron transfer (DET) enzymes containing PEDOT:PSS inks (PEDET ink) and hydrogel precursors inks. The developed spotting techniques allow local deposition of glucose- or lactate-sensitive PEDET inks and hydrogel protection layers on microneedle-based
amperometric sensors. In the Hydrochip2 project, a foil-based microfluidic chip with optical and electrochemical biosensor components for the detection of periodontal germs is investigated. The reagents necessary for the biosensing concepts are incorporated by hydrogel-based reagent reservoirs. These reservoirs are generated and filled with spotting techniques and the release of assay components are studied. In summary, the presentation gives a broad overview of modern techniques for functionalizing POC biosensor systems, which is one of the most important steps towards commercialization.

[1], 01.02.23; [2] Akshat Sudheshwar et al 2023 Flex. Print.
Electron., 8, 015002; [3], 01.02.23
[4], 01.02.23
Zeitraum13 Juni 2023
Ereignistitel3rd International Forum on BioChips & BioChip Solutions
OrtBerlin, Deutschland, BerlinAuf Karte anzeigen

Research Field

  • Molecular Diagnostics


  • Biosensors
  • biofunctionalization
  • dispensing
  • Greensense
  • Hydrochip2