A Fourier ptychographic approach to super-resolution microscopy

Moritz Siegel, Lukas Traxler (Betreuer:in)

Publikation: AbschlussarbeitMasterarbeit

Abstract

In most optic microcopy systems, images are captured using a CCD/CMOS sensor, where the phases of the converted photons are inevitably lost. Fourier ptychographic microscopy (FPM) circumvents this issue by capturing microscopy images illuminated from different angles, and Fourier transforming them computationally (hence the name). Reconstructing the complex object not only yields amplitude but also phase information, enhanced up to super-resolution. Yet one disadvantage remains unsolved: FPM is a very ill-posed problem, the algorithm is not guaranteed to converge to the correct solution, if it converges at all. In practice this means that there is reasonable doubt if the recovered image actually represents the object under the microscope. This work inquires the quality of FPM reconstruction under variation of important system parameters in simulation and experiment. It shows that the alignment of the illumination source is quite critical: Even 0.2 degrees off renders reconstruction useless. This thesis further shows that brightness variations of the individual LEDs are not that important, even assuming unrealistically high deviations, the impact on reconstruction is still negligible. This paper thus furthers the cost-benefit analysis of which amount of computation time should be spent on digital post-correction.
OriginalspracheEnglisch
QualifikationMaster of Science
Gradverleihende Hochschule
  • TU Wien
Betreuer/-in / Berater/-in
  • Schütz, Gerhard, Betreuer:in, Externe Person
  • Traxler, Lukas, Betreuer:in
Datum der Bewilligung16 Mai 2023
DOIs
PublikationsstatusVeröffentlicht - 2023

Research Field

  • High-Performance Vision Systems

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