Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles

Clara-Elvira Pogner, Celia Antunes, Godfrey Apangu, Nicolas Bruffaerts, Sevcan Celenk, Antonella Cristofori, Nestor González Roldán, Agnieszka Grinn-Gofroń, Beatriz Lara, Mirela Lika, Donát Magyar, Moises Martinez-Bracero , Lucia Muggia, Babette Muyshondt, David O'Connor, Alberto Pallavicini, Maria Alexandra Marchã Penha, Rosa Pérez-Badia, Helena Ribeiro, Ana Rodrigues CostaZsófia Tischner, Merita Xhetani, Carsten Ambelas Skjøth

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati
OriginalspracheEnglisch
Aufsatznummer177439
FachzeitschriftScience of the Total Environment
Volume957
DOIs
PublikationsstatusVeröffentlicht - 20 Dez. 2024

Research Field

  • Exploration of Biological Resources

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