TY - JOUR
T1 - Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles
AU - Pogner, Clara-Elvira
AU - Antunes, Celia
AU - Apangu, Godfrey
AU - Bruffaerts, Nicolas
AU - Celenk, Sevcan
AU - Cristofori, Antonella
AU - González Roldán, Nestor
AU - Grinn-Gofroń, Agnieszka
AU - Lara, Beatriz
AU - Lika, Mirela
AU - Magyar, Donát
AU - Martinez-Bracero , Moises
AU - Muggia, Lucia
AU - Muyshondt, Babette
AU - O'Connor, David
AU - Pallavicini, Alberto
AU - Marchã Penha, Maria Alexandra
AU - Pérez-Badia, Rosa
AU - Ribeiro, Helena
AU - Rodrigues Costa, Ana
AU - Tischner, Zsófia
AU - Xhetani, Merita
AU - Ambelas Skjøth, Carsten
PY - 2024/12/20
Y1 - 2024/12/20
N2 - Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati
AB - Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati
KW - Bioaerosols
KW - Molecular genetic analysis
KW - DNA sequencing
KW - Aerobiology
KW - Fungi
KW - Bacteria
KW - Pollen
KW - Virus
U2 - 10.1016/j.scitotenv.2024.177439
DO - 10.1016/j.scitotenv.2024.177439
M3 - Article
SN - 0048-9697
VL - 957
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 177439
ER -