Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles

Clara-Elvira Pogner; Celia Antunes; Godfrey Apangu; Nicolas Bruffaerts; Sevcan Celenk; Antonella Cristofori; Nestor González Roldán; Agnieszka Grinn-Gofroń; Beatriz Lara; Mirela Lika; Donát Magyar; Moises Martinez-Bracero; Lucia Muggia; Babette Muyshondt; David O'Connor; Alberto Pallavicini; Maria Alexandra Marchã Penha; Rosa Pérez-Badia; Helena Ribeiro; Ana Rodrigues Costa; Zsófia Tischner; Merita Xhetani; Carsten Ambelas Skjøth

doi:10.1016/j.scitotenv.2024.177439

Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles

Clara-Elvira Pogner
, Celia Antunes
, Godfrey Apangu
, Nicolas Bruffaerts
, Sevcan Celenk
, Antonella Cristofori
, Nestor González Roldán
, Agnieszka Grinn-Gofroń
, Beatriz Lara
, Mirela Lika
, Donát Magyar
, Moises Martinez-Bracero
, Lucia Muggia
, Babette Muyshondt
, David O'Connor
, Alberto Pallavicini
, Maria Alexandra Marchã Penha
, Rosa Pérez-Badia
, Helena Ribeiro
, Ana Rodrigues Costa

Zsófia Tischner, Merita Xhetani, Carsten Ambelas Skjøth

Bioresources

University of Évora
Rothamsted Research
Sciensano
Bursa Uludağ University
Fondazione Edmund Mach
National Biodiversity Future Center
University of Gothenburg
University of Szczecin
University of Castilla-La Mancha
University of Tirana
National Centre for Public Health and Pharmacy (NCPHP)
University of Córdoba
University of Trieste
Dublin City University
University of Porto
Aarhus University

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

Abstract

Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati

Originalsprache	Englisch
Aufsatznummer	177439
Fachzeitschrift	Science of the Total Environment
Volume	957
DOIs	https://doi.org/10.1016/j.scitotenv.2024.177439
Publikationsstatus	Veröffentlicht - 20 Dez. 2024

Research Field

Exploration of Biological Resources

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10.1016/j.scitotenv.2024.177439

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Pogner, C.-E., Antunes, C., Apangu, G., Bruffaerts, N., Celenk, S., Cristofori, A., González Roldán, N., Grinn-Gofroń, A., Lara, B., Lika, M., Magyar, D., Martinez-Bracero , M., Muggia, L., Muyshondt, B., O'Connor, D., Pallavicini, A., Marchã Penha, M. A., Pérez-Badia, R., Ribeiro, H., ... Ambelas Skjøth, C. (2024). Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles. Science of the Total Environment, 957, Artikel 177439. https://doi.org/10.1016/j.scitotenv.2024.177439

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title = "Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles",

abstract = "Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati",

keywords = "Bioaerosols, Molecular genetic analysis, DNA sequencing, Aerobiology, Fungi, Bacteria, Pollen, Virus",

author = "Clara-Elvira Pogner and Celia Antunes and Godfrey Apangu and Nicolas Bruffaerts and Sevcan Celenk and Antonella Cristofori and \{Gonz{\'a}lez Rold{\'a}n\}, Nestor and Agnieszka Grinn-Gofro{\'n} and Beatriz Lara and Mirela Lika and Don{\'a}t Magyar and Moises Martinez-Bracero and Lucia Muggia and Babette Muyshondt and David O'Connor and Alberto Pallavicini and \{March{\~a} Penha\}, \{Maria Alexandra\} and Rosa P{\'e}rez-Badia and Helena Ribeiro and \{Rodrigues Costa\}, Ana and Zs{\'o}fia Tischner and Merita Xhetani and \{Ambelas Skj{\o}th\}, Carsten",

year = "2024",

month = dec,

day = "20",

doi = "10.1016/j.scitotenv.2024.177439",

language = "English",

volume = "957",

journal = "Science of the Total Environment",

issn = "0048-9697",

publisher = "Elsevier BV",

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Pogner, C-E, Antunes, C, Apangu, G, Bruffaerts, N, Celenk, S, Cristofori, A, González Roldán, N, Grinn-Gofroń, A, Lara, B, Lika, M, Magyar, D, Martinez-Bracero , M, Muggia, L, Muyshondt, B, O'Connor, D, Pallavicini, A, Marchã Penha, MA, Pérez-Badia, R, Ribeiro, H, Rodrigues Costa, A, Tischner, Z, Xhetani, M & Ambelas Skjøth, C 2024, 'Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles', Science of the Total Environment, Vol. 957, 177439. https://doi.org/10.1016/j.scitotenv.2024.177439

TY - JOUR

T1 - Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles

AU - Pogner, Clara-Elvira

AU - Antunes, Celia

AU - Apangu, Godfrey

AU - Bruffaerts, Nicolas

AU - Celenk, Sevcan

AU - Cristofori, Antonella

AU - González Roldán, Nestor

AU - Grinn-Gofroń, Agnieszka

AU - Lara, Beatriz

AU - Lika, Mirela

AU - Magyar, Donát

AU - Martinez-Bracero , Moises

AU - Muggia, Lucia

AU - Muyshondt, Babette

AU - O'Connor, David

AU - Pallavicini, Alberto

AU - Marchã Penha, Maria Alexandra

AU - Pérez-Badia, Rosa

AU - Ribeiro, Helena

AU - Rodrigues Costa, Ana

AU - Tischner, Zsófia

AU - Xhetani, Merita

AU - Ambelas Skjøth, Carsten

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati

AB - Bioaerosol is composed of different particles, originating from organisms, or their fragments with different origin, shape, and size. Sampling, analysing, identification and describing this airborne diversity has been carried out for over 100 years, and more recently the use of molecular genetic tools has been implemented. However, up to now there are no established protocols or standards for detecting airborne diversity of bacteria, fungi, viruses, pollen, and plant particles. In this review we evaluated commonalities of methods used in molecular genetic based studies in the last 23 years, to give an overview of applicable methods as well as knowledge gaps in diversity assessment. Various sampling techniques show different levels of effectiveness in detecting airborne particles based on their DNA. The storage and processing of samples, as well as DNA processing, influences the outcome of sampling campaigns. Moreover, the decisions on barcode selection, method of analysis, reference database as well as negati

KW - Bioaerosols

KW - Molecular genetic analysis

KW - DNA sequencing

KW - Aerobiology

KW - Fungi

KW - Bacteria

KW - Pollen

KW - Virus

U2 - 10.1016/j.scitotenv.2024.177439

DO - 10.1016/j.scitotenv.2024.177439

M3 - Article

SN - 0048-9697

VL - 957

JO - Science of the Total Environment

JF - Science of the Total Environment

M1 - 177439

ER -

Airborne DNA: State of the art – Established methods and missing pieces in the molecular genetic detection of airborne microorganisms, viruses and plant particles

Abstract

Research Field

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