This chapter summarizes some of the efforts in the design, the assembly, the structural and functional characterization, and the sensor application of interfacial layers with molecularly or supramolecularly controlled architectures on solid substrates. It first presents a few considerations for the detection of deoxyribonucleic acid (DNA) amplicons hybridizing from solution to surface-grafted capture oligonucleotide strands. Then, the chapter turns to polymer brushes, functionalized by mouse antibodies. These can be recognized by rat anti-mouse antibodies (RaMABs) carrying a chromophore that can be very sensitively detected by surface plasmon fluorescence spectroscopy (SPFS). It also presents the concept of the tethered bimolecular lipid membrane (t-BLM) as a novel surface-grafted model membrane system that allows for a variety of studies of and with these artificial membranes. The chapter describes the observations of molecular interactions on a metallic surface with controlled interfacial architectures by using combined surface plasmon resonance (SPR) and SPFS.
- Biosensor Technologies
- rat anti-mouse antibodies (RaMABs);