Cell-shaped induced changes of cell functions: controlled cell uptake using ionic biointerfaces

Batirtze Prats Mateu, Peter Ertl, Jose Luis Toca-Herrera

    Publikation: Beitrag in Buch oder TagungsbandBeitrag in Tagungsband mit PosterpräsentationBegutachtung


    In the current project, we have investigated the effect of cationic and anionic interfaces an cell shape and activity. We demonstrate that cellular uptake can be influenced by controlling the biomechanical properties of adherent cells using advanced biointerfaces. Gell biomechanics is an emerging field in cell biology, because it provides insights in cell responses to mechanical stimuli, which is an important parameter known to influence a variety of cell functions (1). For instance, understanding the interdependence between mechanical stimulus, cell shape and function is key in controlling the cell culture microenvironment for pharmacological/medical applications such as the enhancement of transfection of mammalian cells [2]. Our previous results indicated that surface modifications made by th~ Layer-by-Layer technique using different cationic and anionic polyelectrolytes can be used to induce diverse cell shapes thus affecting their biomechanical properties [3]. In this study we have investigated how a shift in biomechanical properties effect cellular activity of various human cell lines including HepG2, NHDF and HUVEC cells. Changes in cell shapes were induced using functionalized glass slides exhibiting either Polyethylenimine (PEI) or Polystyrene sulfonate (PSS) nanolayers. Our results show that the engineered nanolayers of defined structures are able to induce alteration in cell shapes (see Figure 1) and activities. The effects of cell shape on activity and function is demonstrated bY'monitoring cell uptake and internalization of fliiorescent silica particles [4][5][6] and FITC-dextran molecules [7][8] using fluorescent microscopy, flow cytometiy, fluorimetry and confocal microscopy. Additionally, cell-substrate interaction and cell adhesion was characterized using impedance spectroscopy to assess the physiochemical properties of the employed biointerfaces. Overall, the fabrication, characterization and application of ionic interfaces for cell culture handling will be presented to control drug uptake or nutrient release.
    Titel5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013
    PublikationsstatusVeröffentlicht - 2013
    Veranstaltung5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013 -
    Dauer: 24 Sept. 201327 Sept. 2013


    Konferenz5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013

    Research Field

    • Biosensor Technologies


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