Extended lnfectivity Assay - Amplification and Detection of Retroviral Contaminations in a Lab-on-a-Chip

Michaela Purtscher, Mario Rothbauer, Peter Lieberzeit, Andrew Bailey, Peter Ertl

Publikation: Beitrag in Buch oder TagungsbandBeitrag in Tagungsband mit PosterpräsentationBegutachtung

Abstract

retroviral vectors are the most commonly used gene-delivery system in biomedical research due to their stabile integration into the host As genome and inheritance during cell replication. Despite the recently achieved safety improvements using replicaton-defective retroviral vectors still pose a hazard potential of obtaining replication-competent viruses due to recombination events during vector preparation. Consequently, stringent governmental regulations mandate extensive screening for replication-competent retroviruses in all products, vectors and transduced cell lines. Among others cell-based infectivity assays are routinely performed to determine the presence of viral infections or to demonstrate viral clearance. Although cell-based assays are widely used they suffer from poor reproducibility, reliability and are time and labor-intensive. To overcome these technological limitations we have developed a miniaturized and automated a cell-based microsystem consisting of embedded impedance microsensors enabling non-invasive monitoring of virus infections in cell cultures. Our extended infectivity assay is based an simultaneous cellular virus amplification und cellular virus recognition by combining two microfluidic cell cultures an a single chip platform. While virus amplification and release is accomplished by M.dunni resulting viral infection is detected the indicator cell line PG-4. We demonstrated that the integration of two cell cultures in a lab-on-a-chip containing embedded microsensors allows for reliable identification of retrovirus activity within 70 hours using Murine Leukemia Virus based assay.
OriginalspracheEnglisch
Titel5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013
Seiten69
Seitenumfang1
PublikationsstatusVeröffentlicht - 2013
Veranstaltung5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013 -
Dauer: 24 Sept. 201327 Sept. 2013

Konferenz

Konferenz5 th Life Science Meeting Innsbruck, ÖGMBT Jahrestagung 2013
Zeitraum24/09/1327/09/13

Research Field

  • Biosensor Technologies

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