Hepatitis B plasmonic biosensor for the analysis of clinical serum samples

Thomás Riedel, Franti´Sek Surman, Simone Hageneder, Ognen Pop-Georgievski, Christa Nöhammer, Manuela Hofner, Eduard Brynda, Cesar Rodriquez-Emmenegger, Jakub Dostalek

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung


A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
FachzeitschriftBiosensors & Bioelectronics
PublikationsstatusVeröffentlicht - 2016

Research Field

  • Biosensor Technologies


  • Surface plasmon resonance Biosensors
  • Polymer brushes
  • Antifouling


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