Hydrogel-Terminated Photonic Crystal for Label-Free Detection of Angiopoietin-1

Francesca Frascella; Christian Petri; Serena Ricciardi; Lucia Napione; Peter Munzert; Ulrich Jonas; Jakub Dostalek; Federico Bussolino; Candido Pirri; Emiliano Descrovi

doi:10.1109/JLT.2016.2530876

Hydrogel-Terminated Photonic Crystal for Label-Free Detection of Angiopoietin-1

Francesca Frascella, Christian Petri, Serena Ricciardi, Lucia Napione, Peter Munzert, Ulrich Jonas, Jakub Dostalek, Federico Bussolino, Candido Pirri, Emiliano Descrovi

Department of Applied Science and Technology, Polytechnic University of Turin

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

Abstract

We report on a label-free, time-resolved biosensing technique based on refractometric measurements. A onedimensional photonic crystal (1DPC) constituted by a planar stack of dielectric layers is used for supporting Bloch Surface Waves (BSWs). These electromagnetic surface waves probe the aqueous environment within few hundreds of nanometers from the photonic crystal surface, which is functionalized with a hydrogel binding matrix. Therein, selected antibodies are grafted for the specific recognition of the tumoral biomarker Angiopoietin-1. A model bioassay is established demonstrating a detection of Antiopoiegin-1 as low as picomolar concentrations.

Originalsprache	Englisch
Seitenumfang	1
Fachzeitschrift	Journal of Lightwave Technology
Volume	1
Issue	99
DOIs	https://doi.org/10.1109/JLT.2016.2530876
Publikationsstatus	Veröffentlicht - 2016

Research Field

Biosensor Technologies

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title = "Hydrogel-Terminated Photonic Crystal for Label-Free Detection of Angiopoietin-1",

abstract = "We report on a label-free, time-resolved biosensing technique based on refractometric measurements. A onedimensional photonic crystal (1DPC) constituted by a planar stack of dielectric layers is used for supporting Bloch Surface Waves (BSWs). These electromagnetic surface waves probe the aqueous environment within few hundreds of nanometers from the photonic crystal surface, which is functionalized with a hydrogel binding matrix. Therein, selected antibodies are grafted for the specific recognition of the tumoral biomarker Angiopoietin-1. A model bioassay is established demonstrating a detection of Antiopoiegin-1 as low as picomolar concentrations.",

author = "Francesca Frascella and Christian Petri and Serena Ricciardi and Lucia Napione and Peter Munzert and Ulrich Jonas and Jakub Dostalek and Federico Bussolino and Candido Pirri and Emiliano Descrovi",

year = "2016",

doi = "10.1109/JLT.2016.2530876",

language = "English",

volume = "1",

journal = "Journal of Lightwave Technology",

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T1 - Hydrogel-Terminated Photonic Crystal for Label-Free Detection of Angiopoietin-1

AU - Frascella, Francesca

AU - Petri, Christian

AU - Ricciardi, Serena

AU - Napione, Lucia

AU - Munzert, Peter

AU - Jonas, Ulrich

AU - Dostalek, Jakub

AU - Bussolino, Federico

AU - Pirri, Candido

AU - Descrovi, Emiliano

PY - 2016

Y1 - 2016

N2 - We report on a label-free, time-resolved biosensing technique based on refractometric measurements. A onedimensional photonic crystal (1DPC) constituted by a planar stack of dielectric layers is used for supporting Bloch Surface Waves (BSWs). These electromagnetic surface waves probe the aqueous environment within few hundreds of nanometers from the photonic crystal surface, which is functionalized with a hydrogel binding matrix. Therein, selected antibodies are grafted for the specific recognition of the tumoral biomarker Angiopoietin-1. A model bioassay is established demonstrating a detection of Antiopoiegin-1 as low as picomolar concentrations.

AB - We report on a label-free, time-resolved biosensing technique based on refractometric measurements. A onedimensional photonic crystal (1DPC) constituted by a planar stack of dielectric layers is used for supporting Bloch Surface Waves (BSWs). These electromagnetic surface waves probe the aqueous environment within few hundreds of nanometers from the photonic crystal surface, which is functionalized with a hydrogel binding matrix. Therein, selected antibodies are grafted for the specific recognition of the tumoral biomarker Angiopoietin-1. A model bioassay is established demonstrating a detection of Antiopoiegin-1 as low as picomolar concentrations.

U2 - 10.1109/JLT.2016.2530876

DO - 10.1109/JLT.2016.2530876

M3 - Article

SN - 0733-8724

VL - 1

JO - Journal of Lightwave Technology

JF - Journal of Lightwave Technology

IS - 99

ER -

Hydrogel-Terminated Photonic Crystal for Label-Free Detection of Angiopoietin-1

Abstract

Research Field

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