Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS)

Renate L. C. Naumann, Vedran Nedelkovski, Andreas Schwaighofer (Vortragende:r), C. Wraight, Anthony Crofts, Christoph Nowak

Publikation: Beitrag in Buch oder TagungsbandBeitrag in Tagungsband mit PosterpräsentationBegutachtung

Abstract

Co-reconstitution of photosynthetic reaction centers(RCs) with bc1 complexes, both from Rb. Sphaeroides has been investigated using surface-enhanced IR-absorption spectroscopy (SEIRAS) in the ATR configuration. Surface-enhancement is achieved by a thin nano-structured gold film present on the surface of an ATR crystal. The proteins were simultaneously immobilized as a monolayer on the gold film via His-tag technology and subsequently co-reconstituted by in-situ dialysis into a lipid bilayer. Spectra were recorded as a function of time under continuous illumination with white light from a Fiber-Lite DC950 illuminator (150 W, quartz halogen lamp).
OriginalspracheEnglisch
Titel57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania
PublikationsstatusVeröffentlicht - 2013
Veranstaltung57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania -
Dauer: 2 Feb. 20136 Feb. 2013

Konferenz

Konferenz57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania
Zeitraum2/02/136/02/13

Research Field

  • Biosensor Technologies

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