Ligand-binding assays with OBPs and CSPs

Chiara D´Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll, Paolo Pelosi

    Publikation: Beitrag in Buch oder TagungsbandBuchkapitel


    Assessing the ligand-binding properties of OBPs and CSPs is essential for understanding their physiological function. It also provides basic information when these proteins are used as biosensing elements for instrumental measurement of odors. Although different approaches have been applied in the past to evaluate the affinity of receptors and soluble binding proteins to their ligands, using a fluorescent reporter represents the method of choice for OBPs and CSPs. It offers the advantages of working at the equilibrium, being simple, fast and inexpensive, without requiring the use of radioactive tracers. However, as an indirect method, the fluorescence competitive binding approach presents drawbacks and sometimes requires an elaborate analysis to explain unexpected results. Here, after a brief survey of the different approaches to evaluate affinity constants, we focus on the fluorescence binding assay as applied to OBPs and CSPs, discussing situations that may require closer inspection of the results.
    TitelMethods in Enzymology
    Redakteure/-innenPaolo Pelosi, Wolfgang Knoll
    Herausgeber (Verlag)Elsevier
    PublikationsstatusVeröffentlicht - 2020

    Research Field

    • Biosensor Technologies


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