Matrix Metalloproteinase Sensing in Wound Fluids: Are Graphene-Based Field Effect Transistors a Viable Alternative?

Teresa Isabel Loureiro Fidalgo do Vale Rodrigues, Adrien Hugo, Julia Mader, Wolfgang Knoll, Vincent Bouchiat, Rabah Boukherroub, Sabine Szunerits

    Publikation: Beitrag in FachzeitschriftArtikel

    Abstract

    Wound dressings based on graphene sheets are expected to play a crucial role in wound treatment. The good conductivity and charge mobility of graphene as an electronic material makes graphene-based field effect transistors well-adapted for sensing of wound biomarkers. In this work, an aptamer-based field effect transistor using graphene as sensing channel material was developed and evaluated for the selective and sensitive monitoring of matrix metalloproteinases 9 (MMP-9), a wound biomarker upregulated in non-healing wounds. Concentration-dependent measurements performed with wound fluid spiked with MMP-9 on GFET-MMP/PEG sensors exhibited real-time detection of MMP-9 with a detection limit of 96 pM in 1x MOPS/1 mM CaCl 2. The sensor was tested on swab samples from non-healing wounds and compared to results of antibody-based ELISA analysis. In contrast to ELISA, the GFET sensor was only sensing the active MMP-9 form and not the mixture of active and inactive forms, as evidenced by the analysis performed in the presence of EDTA as inactivation medium.
    OriginalspracheEnglisch
    Seitenumfang1
    FachzeitschriftSSRN Electronic Journal
    Volume1
    DOIs
    PublikationsstatusVeröffentlicht - 2022

    Research Field

    • Biosensor Technologies

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