Nanozyme activity of platinum nanoparticles prepared by pulsed laser ablation

Steffen Kurzhals (Autor:in und Vortragende:r), Eva Melnik, Mariangela Fedel, Laszlo Sajti, Rainer Hainberger

Publikation: Beitrag in Buch oder TagungsbandBeitrag in Tagungsband mit PosterpräsentationBegutachtung

Abstract

Platinum nanoparticles (Pt-NPs) were generated by pulsed laser ablation in liquid and
proved to be stable in aqueous dispersion for at least six months when stabilized by small amount of
sodium citrate. Their nanozyme activity was tested photometrically with tetramethylbenzidine (TMB) in
the absence and presence of hydrogen peroxide (H2O2) at 22°C, which lead to specific activities (SA)
of 1.9U/mg Pt-NPs and 375U/mg Pt-NPs and limits of detection (LOD) of 1.86pmol/L and
0.026pmol/L, respectively. In comparison, a SA of 289U/mg and a LOD of 36pmol/L were determined
for a recombinant horseradish peroxidase in presence of H2O2 at 22°C. In particular, the lower detection
limit of Pt-NPs by a factor of 1384 compared to the recombinant horseradish peroxidase indicates
their great potential for future bioassays.
OriginalspracheEnglisch
TitelEUROSENSORS XXXVI
KapitelPT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems
Seiten425 - 426
Seitenumfang2
ISBN (elektronisch)978-3-910600-03-4
DOIs
PublikationsstatusVeröffentlicht - 16 Sept. 2024

Publikationsreihe

NamePoster

Research Field

  • Molecular Diagnostics

Schlagwörter

  • nanoparticle
  • nanozyme
  • Activity
  • Assays

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