Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.