Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Naoto Asai (Vortragende:r), Gizem Aktug, Andres de los Santos, Thomás Riedel, Jakub Dostalek

    Publikation: Beitrag in Buch oder TagungsbandVortrag mit Beitrag in TagungsbandBegutachtung

    Abstract

    To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.
    OriginalspracheEnglisch
    TitelPROCEEDINGS VOLUME 12572
    Redakteure/-innenFrancesco Baldini, Jiri Homola, Robert A. Lieberman
    Band12572
    DOIs
    PublikationsstatusVeröffentlicht - 31 Mai 2023
    VeranstaltungSPIE Optics + Optoelectronics: Optical Sensors 2023 - Prague, Tschechische Republik
    Dauer: 24 Apr. 202327 Apr. 2023
    Konferenznummer: 12572
    https://spie.org/conferences-and-exhibitions/optics-and-optoelectronics

    Publikationsreihe

    NameOptical Sensors 2023

    Konferenz

    KonferenzSPIE Optics + Optoelectronics
    Land/GebietTschechische Republik
    StadtPrague
    Zeitraum24/04/2327/04/23
    Internetadresse

    Research Field

    • Biosensor Technologies

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