TY - JOUR
T1 - Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides
AU - Schwaighofer, Andreas
AU - Steininger, Christoph
AU - Hildenbrandt, M. David
AU - Srajer, Johannes
AU - Nowak, Christoph
AU - Knoll, Wolfgang
AU - Naumann, Renate L. C.
PY - 2013
Y1 - 2013
N2 - Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
AB - Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
U2 - 10.1016/j.bpj.2013.10.037
DO - 10.1016/j.bpj.2013.10.037
M3 - Article
SN - 0006-3495
VL - 105
SP - 2706
EP - 2713
JO - Biophysical Journal
JF - Biophysical Journal
ER -