Description
Periodontitis is a chronic inflammatory disease and the main cause of tooth loss. It is caused by infection with gram-positive and gram-negative bacteria. For therapy it is recommended to adjust the medication to the causative pathogens and to control the therapy response in short time intervals. Although various diagnostic systems are currently on the market, the complex handling, the long analysis times and/or the high acquisition and operating costs prevent the widespread use of these systems.In the HydroChip2 project (FFG No. 883914), we are addressing this need by developing a foil-based rapid test system for the detection of nucleic acids for the identification of periodontal pathogens. The development steps include 1) the realisation of a novel bacterial lysis based on ionic liquids [1] at room temperature, 2) the reduction of handling complexity by introducing hydrogel reagent reservoirs and 3) the realisation of bacterial identification by detecting ribosomal RNA using different analytical methods, namely chemiluminescence (CL), electrochemical (EC) detection [2] and electrochemiluminescence (ECL). While CL is the state of the art, microfluidic integrated EC and ECL are very promising methods to reduce system complexity and costs, as well as to increase sensitivity.
The processes for the successful implementation of reagent reservoirs in the microfluidic channels using upscale-able printing technologies will be discussed and the feasibility shown for the total germ load (E. coli) detection.
References
[1] Martzy, Roland; Bica-Schröder, Katharina; Pálvölgyi, Ádám Márk; Kolm, Claudia; Jakwerth, Stefan; Kirschner, Alexander K. T. et al. (2019): Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids. In: Scientific reports 9 (1), S. 13994.
| Period | 14 May 2024 |
|---|---|
| Event title | 8th International Conference on bio-Sensing Technology |
| Event type | Conference |
| Location | Seville, SpainShow on map |
Research Field
- Molecular Diagnostics