Abstract
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate) (PST-co-VBT). Both ARChip surfaces are tested in a model assay based on oligonucleotide probes from a real-life genotyping project and are evaluated in comparison with five commercial chip surfaces based on nitrocellulose, epoxy- and aldehyde-polymer, and two different aminosilanes. Optimum print buffer, spotter compatibility and data normalization are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 29-36 |
| Number of pages | 8 |
| Journal | Analytical Biochemistry |
| Publication status | Published - 2004 |
Research Field
- Not defined
Keywords
- covalent immobilization
- oligonucleotide
- epoxy resin
- vinylbenzylthiocyanate
- biochip
- methanotrophic bacteria