Biosensor Based on Hydrogel Optical Waveguide Spectroscopy for the Detection of 17β-Estradiol

Qingwen Zhang; Yi Wang; Anca Mateescu; Khulan Sergelen; Asmorom Kibrom; Ulrich Jonas; Tianxin Wei; Jakub Dostalek

doi:10.1016/j.talanta.2012.11.017

Biosensor Based on Hydrogel Optical Waveguide Spectroscopy for the Detection of 17β-Estradiol

Qingwen Zhang
, Yi Wang
, Anca Mateescu
, Khulan Sergelen
, Asmorom Kibrom
, Ulrich Jonas
, Tianxin Wei
, Jakub Dostalek

Research output: Contribution to journal › Article › peer-review

Abstract

Rapid detectionofhormonesatsub-ng/mlconcentrationsisoftremendousimportancefordiagnostic purposes,qualitycontrol,andenvironmentalmonitoring.Inthisrespect,wereportanovellabel-free biosensorbasedonhydrogelopticalwaveguidespectroscopy(HOWS)forthesensitivedetectionof 17b-estradiol(E2).Thisapproachwasimplementedbyusingathinhydrogellayerofacarboxylated poly(N-isoproprylacrylamide)(PNIPAAm)terpolymerthatwasattachedtoametallicsensorsurfacein order tosimultaneouslyserveasabindingmatrixandanopticalwaveguide.Refractiveindexchanges that areaccompaniedwiththespecificcaptureofbiomoleculesfromanaqueoussampleonthesensor surface wereprobedbyresonantlyexcitedhydrogelopticalwaveguidemodes.Toopticallyexciteand interrogatethesewaves,anopticalsetupbasedonKretschmannconfigurationofattenuatedtotal reflection(ATR)methodthatiscompatiblewithsurfaceplasmonresonance(SPR)wasused.We demonstratethatHOWSoffersahigherbindingcapacity,goodanti-foulingproperties,improvedfigure of merit,andE2detectionlimitof50pg/mlwhichisseventimesbetterthanthatobtainedbyaregular surface plasmonresonance(SPR)biosensor.

Original language	English
Pages (from-to)	149-154
Number of pages	6
Journal	Talanta
Volume	104
DOIs	https://doi.org/10.1016/j.talanta.2012.11.017
Publication status	Published - 2012

Research Field

Outside the AIT Research Fields

Keywords

Surface plasmonresonance (SPR)
Hydrogel opticalwaveguide (HOW)
Hydrogel binding matrix
17b-estradiol (E2)
Biosensors

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@article{16b48967c6924391afc5d6be9e541209,

title = "Biosensor Based on Hydrogel Optical Waveguide Spectroscopy for the Detection of 17β-Estradiol",

abstract = "Rapid detectionofhormonesatsub-ng/mlconcentrationsisoftremendousimportancefordiagnostic purposes,qualitycontrol,andenvironmentalmonitoring.Inthisrespect,wereportanovellabel-free biosensorbasedonhydrogelopticalwaveguidespectroscopy(HOWS)forthesensitivedetectionof 17b-estradiol(E2).Thisapproachwasimplementedbyusingathinhydrogellayerofacarboxylated poly(N-isoproprylacrylamide)(PNIPAAm)terpolymerthatwasattachedtoametallicsensorsurfacein order tosimultaneouslyserveasabindingmatrixandanopticalwaveguide.Refractiveindexchanges that areaccompaniedwiththespecificcaptureofbiomoleculesfromanaqueoussampleonthesensor surface wereprobedbyresonantlyexcitedhydrogelopticalwaveguidemodes.Toopticallyexciteand interrogatethesewaves,anopticalsetupbasedonKretschmannconfigurationofattenuatedtotal reflection(ATR)methodthatiscompatiblewithsurfaceplasmonresonance(SPR)wasused.We demonstratethatHOWSoffersahigherbindingcapacity,goodanti-foulingproperties,improvedfigure of merit,andE2detectionlimitof50pg/mlwhichisseventimesbetterthanthatobtainedbyaregular surface plasmonresonance(SPR)biosensor.",

keywords = "Surface plasmonresonance (SPR), Hydrogel opticalwaveguide (HOW), Hydrogel binding matrix, 17b-estradiol (E2), Biosensors, Surface plasmonresonance (SPR), Hydrogel opticalwaveguide (HOW), Hydrogel binding matrix, 17b-estradiol (E2), Biosensors",

author = "Qingwen Zhang and Yi Wang and Anca Mateescu and Khulan Sergelen and Asmorom Kibrom and Ulrich Jonas and Tianxin Wei and Jakub Dostalek",

year = "2012",

doi = "10.1016/j.talanta.2012.11.017",

language = "English",

volume = "104",

pages = "149--154",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Biosensor Based on Hydrogel Optical Waveguide Spectroscopy for the Detection of 17β-Estradiol

AU - Zhang, Qingwen

AU - Wang, Yi

AU - Mateescu, Anca

AU - Sergelen, Khulan

AU - Kibrom, Asmorom

AU - Jonas, Ulrich

AU - Wei, Tianxin

AU - Dostalek, Jakub

PY - 2012

Y1 - 2012

N2 - Rapid detectionofhormonesatsub-ng/mlconcentrationsisoftremendousimportancefordiagnostic purposes,qualitycontrol,andenvironmentalmonitoring.Inthisrespect,wereportanovellabel-free biosensorbasedonhydrogelopticalwaveguidespectroscopy(HOWS)forthesensitivedetectionof 17b-estradiol(E2).Thisapproachwasimplementedbyusingathinhydrogellayerofacarboxylated poly(N-isoproprylacrylamide)(PNIPAAm)terpolymerthatwasattachedtoametallicsensorsurfacein order tosimultaneouslyserveasabindingmatrixandanopticalwaveguide.Refractiveindexchanges that areaccompaniedwiththespecificcaptureofbiomoleculesfromanaqueoussampleonthesensor surface wereprobedbyresonantlyexcitedhydrogelopticalwaveguidemodes.Toopticallyexciteand interrogatethesewaves,anopticalsetupbasedonKretschmannconfigurationofattenuatedtotal reflection(ATR)methodthatiscompatiblewithsurfaceplasmonresonance(SPR)wasused.We demonstratethatHOWSoffersahigherbindingcapacity,goodanti-foulingproperties,improvedfigure of merit,andE2detectionlimitof50pg/mlwhichisseventimesbetterthanthatobtainedbyaregular surface plasmonresonance(SPR)biosensor.

AB - Rapid detectionofhormonesatsub-ng/mlconcentrationsisoftremendousimportancefordiagnostic purposes,qualitycontrol,andenvironmentalmonitoring.Inthisrespect,wereportanovellabel-free biosensorbasedonhydrogelopticalwaveguidespectroscopy(HOWS)forthesensitivedetectionof 17b-estradiol(E2).Thisapproachwasimplementedbyusingathinhydrogellayerofacarboxylated poly(N-isoproprylacrylamide)(PNIPAAm)terpolymerthatwasattachedtoametallicsensorsurfacein order tosimultaneouslyserveasabindingmatrixandanopticalwaveguide.Refractiveindexchanges that areaccompaniedwiththespecificcaptureofbiomoleculesfromanaqueoussampleonthesensor surface wereprobedbyresonantlyexcitedhydrogelopticalwaveguidemodes.Toopticallyexciteand interrogatethesewaves,anopticalsetupbasedonKretschmannconfigurationofattenuatedtotal reflection(ATR)methodthatiscompatiblewithsurfaceplasmonresonance(SPR)wasused.We demonstratethatHOWSoffersahigherbindingcapacity,goodanti-foulingproperties,improvedfigure of merit,andE2detectionlimitof50pg/mlwhichisseventimesbetterthanthatobtainedbyaregular surface plasmonresonance(SPR)biosensor.

KW - Surface plasmonresonance (SPR)

KW - Hydrogel opticalwaveguide (HOW)

KW - Hydrogel binding matrix

KW - 17b-estradiol (E2)

KW - Biosensors

KW - Surface plasmonresonance (SPR)

KW - Hydrogel opticalwaveguide (HOW)

KW - Hydrogel binding matrix

KW - 17b-estradiol (E2)

KW - Biosensors

U2 - 10.1016/j.talanta.2012.11.017

DO - 10.1016/j.talanta.2012.11.017

M3 - Article

SN - 0039-9140

VL - 104

SP - 149

EP - 154

JO - Talanta

JF - Talanta

ER -

Biosensor Based on Hydrogel Optical Waveguide Spectroscopy for the Detection of 17β-Estradiol

Abstract

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Keywords

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