Electron Transfer to Light-Activated Photosynthetic Reaction Centers from Rhodobacter sphaeroides Reconstituted in a Biomimetic Membrane System

Jens Gebert; Ciril Rozman; Christoph Steininger; Vedran Nedelkovski; Christoph Nowak; C. Wraight; Renate L. C. Naumann

doi:10.1021/jp510006n

Electron Transfer to Light-Activated Photosynthetic Reaction Centers from Rhodobacter sphaeroides Reconstituted in a Biomimetic Membrane System

Jens Gebert, Ciril Rozman, Christoph Steininger, Vedran Nedelkovski, Christoph Nowak, C. Wraight, Renate L. C. Naumann

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Abstract

Photosynthetic reaction centers (RCs) from Rb. sphaeroides with a genetically engineered 7-his-tag at the C-terminus of the M-subunit are bound to a Ni-NTA-modified gold surface. Subsequently, the bound RCs are subjected to in situ dialysis in the presence of lipid micelles to form a protein-tethered lipid bilayer membrane (ptBLM). Redox properties of the RC thus immobilized are investigated by cyclic voltammetry. Photocurrrents are generated in the range of 10 μA cm−2, however, different from previous studies at potentials of −200 and −300 mV, and without cytochrome c as a mediator. The unexpected behavior is explained in terms of an interprotein reaction between RC molecules promoted by the lipid bilayer, which we had previously detected by surface-enhanced infrared absorption spectroscopy.

Original language	English
Pages (from-to)	890-895
Number of pages	6
Journal	Journal of Physical Chemistry C
Volume	119
DOIs	https://doi.org/10.1021/jp510006n
Publication status	Published - 2015

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title = "Electron Transfer to Light-Activated Photosynthetic Reaction Centers from Rhodobacter sphaeroides Reconstituted in a Biomimetic Membrane System",

abstract = "Photosynthetic reaction centers (RCs) from Rb. sphaeroides with a genetically engineered 7-his-tag at the C-terminus of the M-subunit are bound to a Ni-NTA-modified gold surface. Subsequently, the bound RCs are subjected to in situ dialysis in the presence of lipid micelles to form a protein-tethered lipid bilayer membrane (ptBLM). Redox properties of the RC thus immobilized are investigated by cyclic voltammetry. Photocurrrents are generated in the range of 10 μA cm−2, however, different from previous studies at potentials of −200 and −300 mV, and without cytochrome c as a mediator. The unexpected behavior is explained in terms of an interprotein reaction between RC molecules promoted by the lipid bilayer, which we had previously detected by surface-enhanced infrared absorption spectroscopy.",

author = "Jens Gebert and Ciril Rozman and Christoph Steininger and Vedran Nedelkovski and Christoph Nowak and C. Wraight and Naumann, {Renate L. C.}",

year = "2015",

doi = "10.1021/jp510006n",

language = "English",

volume = "119",

pages = "890--895",

journal = "Journal of Physical Chemistry C",

issn = "1932-7447",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Electron Transfer to Light-Activated Photosynthetic Reaction Centers from Rhodobacter sphaeroides Reconstituted in a Biomimetic Membrane System

AU - Gebert, Jens

AU - Rozman, Ciril

AU - Steininger, Christoph

AU - Nedelkovski, Vedran

AU - Nowak, Christoph

AU - Wraight, C.

AU - Naumann, Renate L. C.

PY - 2015

Y1 - 2015

N2 - Photosynthetic reaction centers (RCs) from Rb. sphaeroides with a genetically engineered 7-his-tag at the C-terminus of the M-subunit are bound to a Ni-NTA-modified gold surface. Subsequently, the bound RCs are subjected to in situ dialysis in the presence of lipid micelles to form a protein-tethered lipid bilayer membrane (ptBLM). Redox properties of the RC thus immobilized are investigated by cyclic voltammetry. Photocurrrents are generated in the range of 10 μA cm−2, however, different from previous studies at potentials of −200 and −300 mV, and without cytochrome c as a mediator. The unexpected behavior is explained in terms of an interprotein reaction between RC molecules promoted by the lipid bilayer, which we had previously detected by surface-enhanced infrared absorption spectroscopy.

AB - Photosynthetic reaction centers (RCs) from Rb. sphaeroides with a genetically engineered 7-his-tag at the C-terminus of the M-subunit are bound to a Ni-NTA-modified gold surface. Subsequently, the bound RCs are subjected to in situ dialysis in the presence of lipid micelles to form a protein-tethered lipid bilayer membrane (ptBLM). Redox properties of the RC thus immobilized are investigated by cyclic voltammetry. Photocurrrents are generated in the range of 10 μA cm−2, however, different from previous studies at potentials of −200 and −300 mV, and without cytochrome c as a mediator. The unexpected behavior is explained in terms of an interprotein reaction between RC molecules promoted by the lipid bilayer, which we had previously detected by surface-enhanced infrared absorption spectroscopy.

U2 - 10.1021/jp510006n

DO - 10.1021/jp510006n

M3 - Article

SN - 1932-7447

VL - 119

SP - 890

EP - 895

JO - Journal of Physical Chemistry C

JF - Journal of Physical Chemistry C

ER -

Electron Transfer to Light-Activated Photosynthetic Reaction Centers from Rhodobacter sphaeroides Reconstituted in a Biomimetic Membrane System

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