Establishment of Vascular Networks in Biochips Using Co-cultures of Adipose Derived Stem Cells and Endothelial Cells in a 3D Fibrin Matrix

Michaela Purtscher, Mario Rothbauer, Heinz Redl, Peter Ertl

    Research output: Chapter in Book or Conference ProceedingsConference Proceedings without Presentationpeer-review

    Abstract

    The development of novel vascularization strategies for tissue constructs is still one of the major challenges in tissue engineering and regeneration. In the present study we investigate the establishment of vascular networks in the absence and presence of fluid flow conditions using a microfluidic co-culture system. In the presented configuration adipose derived stem cells (ASCs) and human umbilical vein cells (HUVEC) are embedded within a 3D fibrin matrix to study accumulation and depletion of ASCs secreted paracrine pro-angiogenic factors on vascular tube formation. Initial experiments using fluorescently labelled bovine serum albumin confirmed rapid transport of biomolecules within the fibrin matrix over a distance of various millimeters. Results using 3D co-cultures showed that even in the presence of low fluid mechanical shear forces of 0.025 dyn/mm2 the onset and maturation of vascular endothelial networks formation were significantly affected, leading to altered tube size and tube length.
    Original languageEnglish
    Title of host publication6th European Conference of the International Federation for Medical and Biological Engineering
    Pages313-317
    Number of pages5
    DOIs
    Publication statusPublished - 2015

    Research Field

    • Biosensor Technologies

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