Hepatitis B plasmonic biosensor for the analysis of clinical serum samples

Thomás Riedel; Franti´Sek Surman; Simone Hageneder; Ognen Pop-Georgievski; Christa Nöhammer; Manuela Hofner; Eduard Brynda; Cesar Rodriquez-Emmenegger; Jakub Dostalek

doi:10.1016/j.bios.2016.05.014

Hepatitis B plasmonic biosensor for the analysis of clinical serum samples

Thomás Riedel, Franti´Sek Surman, Simone Hageneder, Ognen Pop-Georgievski, Christa Nöhammer, Manuela Hofner, Eduard Brynda, Cesar Rodriquez-Emmenegger, Jakub Dostalek

Molecular Diagnostics

Research output: Contribution to journal › Article › peer-review

Abstract

A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.

Original language	English
Number of pages	5
Journal	Biosensors & Bioelectronics
Volume	85
Issue number	85
DOIs	https://doi.org/10.1016/j.bios.2016.05.014
Publication status	Published - 2016

Research Field

Biosensor Technologies

Keywords

Surface plasmon resonance Biosensors
Polymer brushes
Antifouling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2016.05.014Licence: Unspecified

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title = "Hepatitis B plasmonic biosensor for the analysis of clinical serum samples",

abstract = "A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.",

keywords = "Surface plasmon resonance Biosensors, Polymer brushes, Antifouling, Surface plasmon resonance Biosensors, Polymer brushes, Antifouling",

author = "Thom{\'a}s Riedel and Franti´Sek Surman and Simone Hageneder and Ognen Pop-Georgievski and Christa N{\"o}hammer and Manuela Hofner and Eduard Brynda and Cesar Rodriquez-Emmenegger and Jakub Dostalek",

year = "2016",

doi = "10.1016/j.bios.2016.05.014",

language = "English",

volume = "85",

journal = "Biosensors & Bioelectronics",

issn = "0956-5663",

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T1 - Hepatitis B plasmonic biosensor for the analysis of clinical serum samples

AU - Riedel, Thomás

AU - Surman, Franti´Sek

AU - Hageneder, Simone

AU - Pop-Georgievski, Ognen

AU - Nöhammer, Christa

AU - Hofner, Manuela

AU - Brynda, Eduard

AU - Rodriquez-Emmenegger, Cesar

AU - Dostalek, Jakub

PY - 2016

Y1 - 2016

N2 - A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.

AB - A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.

KW - Surface plasmon resonance Biosensors

KW - Polymer brushes

KW - Antifouling

KW - Surface plasmon resonance Biosensors

KW - Polymer brushes

KW - Antifouling

U2 - 10.1016/j.bios.2016.05.014

DO - 10.1016/j.bios.2016.05.014

M3 - Article

SN - 0956-5663

VL - 85

JO - Biosensors & Bioelectronics

JF - Biosensors & Bioelectronics

IS - 85

ER -

Hepatitis B plasmonic biosensor for the analysis of clinical serum samples

Abstract

Research Field

Keywords

UN SDGs

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