Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS)

Renate L. C. Naumann; Vedran Nedelkovski; Andreas Schwaighofer; C. Wraight; Anthony Crofts; Christoph Nowak

Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS)

Renate L. C. Naumann
, Vedran Nedelkovski
, Andreas Schwaighofer (Speaker)
, C. Wraight
, Anthony Crofts
, Christoph Nowak

Research output: Chapter in Book or Conference Proceedings › Conference Proceedings with Poster Presentation › peer-review

Abstract

Co-reconstitution of photosynthetic reaction centers(RCs) with bc1 complexes, both from Rb. Sphaeroides has been investigated using surface-enhanced IR-absorption spectroscopy (SEIRAS) in the ATR configuration. Surface-enhancement is achieved by a thin nano-structured gold film present on the surface of an ATR crystal. The proteins were simultaneously immobilized as a monolayer on the gold film via His-tag technology and subsequently co-reconstituted by in-situ dialysis into a lipid bilayer. Spectra were recorded as a function of time under continuous illumination with white light from a Fiber-Lite DC950 illuminator (150 W, quartz halogen lamp).

Original language	English
Title of host publication	57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania
Publication status	Published - 2013
Event	57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania - Duration: 2 Feb 2013 → 6 Feb 2013

Conference

Conference	57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania
Period	2/02/13 → 6/02/13

Research Field

Biosensor Technologies

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Naumann, R. L. C., Nedelkovski, V., Schwaighofer, A., Wraight, C., Crofts, A., & Nowak, C. (2013). Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS). In 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania

Naumann, Renate L. C. ; Nedelkovski, Vedran ; Schwaighofer, Andreas et al. / Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS). 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania. 2013.

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title = "Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS)",

abstract = "Co-reconstitution of photosynthetic reaction centers(RCs) with bc1 complexes, both from Rb. Sphaeroides has been investigated using surface-enhanced IR-absorption spectroscopy (SEIRAS) in the ATR configuration. Surface-enhancement is achieved by a thin nano-structured gold film present on the surface of an ATR crystal. The proteins were simultaneously immobilized as a monolayer on the gold film via His-tag technology and subsequently co-reconstituted by in-situ dialysis into a lipid bilayer. Spectra were recorded as a function of time under continuous illumination with white light from a Fiber-Lite DC950 illuminator (150 W, quartz halogen lamp).",

author = "Naumann, \{Renate L. C.\} and Vedran Nedelkovski and Andreas Schwaighofer and C. Wraight and Anthony Crofts and Christoph Nowak",

year = "2013",

language = "English",

booktitle = "57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania",

note = "57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania ; Conference date: 02-02-2013 Through 06-02-2013",

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Naumann, RLC, Nedelkovski, V, Schwaighofer, A, Wraight, C, Crofts, A & Nowak, C 2013, Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS). in 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania. 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania, 2/02/13.

Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS). / Naumann, Renate L. C.; Nedelkovski, Vedran; Schwaighofer, Andreas (Speaker) et al.
57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania. 2013.

Research output: Chapter in Book or Conference Proceedings › Conference Proceedings with Poster Presentation › peer-review

TY - CHAP

T1 - Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS)

AU - Naumann, Renate L. C.

AU - Nedelkovski, Vedran

AU - Wraight, C.

AU - Crofts, Anthony

AU - Nowak, Christoph

A2 - Schwaighofer, Andreas

PY - 2013

Y1 - 2013

N2 - Co-reconstitution of photosynthetic reaction centers(RCs) with bc1 complexes, both from Rb. Sphaeroides has been investigated using surface-enhanced IR-absorption spectroscopy (SEIRAS) in the ATR configuration. Surface-enhancement is achieved by a thin nano-structured gold film present on the surface of an ATR crystal. The proteins were simultaneously immobilized as a monolayer on the gold film via His-tag technology and subsequently co-reconstituted by in-situ dialysis into a lipid bilayer. Spectra were recorded as a function of time under continuous illumination with white light from a Fiber-Lite DC950 illuminator (150 W, quartz halogen lamp).

AB - Co-reconstitution of photosynthetic reaction centers(RCs) with bc1 complexes, both from Rb. Sphaeroides has been investigated using surface-enhanced IR-absorption spectroscopy (SEIRAS) in the ATR configuration. Surface-enhancement is achieved by a thin nano-structured gold film present on the surface of an ATR crystal. The proteins were simultaneously immobilized as a monolayer on the gold film via His-tag technology and subsequently co-reconstituted by in-situ dialysis into a lipid bilayer. Spectra were recorded as a function of time under continuous illumination with white light from a Fiber-Lite DC950 illuminator (150 W, quartz halogen lamp).

M3 - Conference Proceedings with Poster Presentation

BT - 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania

T2 - 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania

Y2 - 2 February 2013 through 6 February 2013

ER -

Naumann RLC, Nedelkovski V, Schwaighofer A, Wraight C, Crofts A, Nowak C. Interaction of light-activated Photosynthetic Reaction Centers co-reconstituted in a strict orientation with the bc1 complex from Rhodobacter sphaeroides into a lipid bilayer followed by Surface-Enhanced Infrared-Absorption Spectroscopy (SEIRAS). In 57 th Annual Meeting Biophysical Society, Philadelphia, Pennsylvania. 2013