Methods: The sample cohort underlying this study consist of whole blood, plasma and cell-free saliva collected from a total of 157 patients, which had undergone CA. 84 of these patients showed significant stenosis, which was defined as > 50% lumen narrowing in at least one coronary artery. We performed biomarker discovery studies with about 30-40 patients per experimental group and omics layer. The discovery on the 3 omics layers was conducted via A) genome-wide DNA methylation profiling from whole blood on Illumina EPIC microarrays, B) proteome-wide antibody profiling from plasma on in-house produced high density protein microarrays and C) small RNA sequencing from plasma- and cell-free saliva-derived extracellular vesicles.
Results: The current preliminary data showed a variety of statistically significant differences in DNA-methylation-, small RNA and autoantibody profiles between stenosis and non-stenosis patients.
Conclusion: Multi-omics profiling from liquid biopsies such as blood and saliva is a promising approach to diagnose and give insight into complex diseases such as CAD and cancer.
|Title of host publication
|Tagungsband Leibnitz Conference
|Subtitle of host publication
|30. Leibnitz Conference of Advance Science, Berlin Oct. 6th, 2023
|Number of pages
|Published - 5 Oct 2023
- Molecular Diagnostics