New alleles and mutational events at 14 STR loci from different German populations

Dorit Becker; Klaus Bender; Jeanett Edelmann; Frank Götz; Lotte Henke; Sandra Hering; Carsten Hohoff; Karolin Hoppe; Michael Klintschar; Matthias Muche; Burkhard Rolf; Reinhard Szibor; Volker Weirich; Martin Jung; Werner Brabetz

doi:10.1016/j.fsigen.2007.04.001

New alleles and mutational events at 14 STR loci from different German populations

Dorit Becker, Klaus Bender, Jeanett Edelmann, Frank Götz, Lotte Henke, Sandra Hering, Carsten Hohoff, Karolin Hoppe, Michael Klintschar, Matthias Muche, Burkhard Rolf, Reinhard Szibor, Volker Weirich, Martin Jung, Werner Brabetz

Molecular Diagnostics

Biotype AG

Research output: Contribution to journal › Article › peer-review

Abstract

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.

Original language	English
Pages (from-to)	232-7
Number of pages	6
Journal	Forensic Science International: Genetics
Volume	1
Issue number	3-4
DOIs	https://doi.org/10.1016/j.fsigen.2007.04.001
Publication status	Published - Dec 2007

Research Field

Molecular Diagnostics

Keywords

Alleles
Base Sequence
DNA/genetics
DNA Primers/genetics
Female
Forensic Genetics
Gene Frequency
Genetics, Population
Germany
Humans
Male
Microsatellite Repeats
Mutation

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10.1016/j.fsigen.2007.04.001

Cite this

Becker, D., Bender, K., Edelmann, J., Götz, F., Henke, L., Hering, S., Hohoff, C., Hoppe, K., Klintschar, M., Muche, M., Rolf, B., Szibor, R., Weirich, V., Jung, M., & Brabetz, W. (2007). New alleles and mutational events at 14 STR loci from different German populations. Forensic Science International: Genetics, 1(3-4), 232-7. https://doi.org/10.1016/j.fsigen.2007.04.001

@article{2eb949d544b0415aa2c7eb1a97a7b01f,

title = "New alleles and mutational events at 14 STR loci from different German populations",

abstract = "The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.",

keywords = "Alleles, Base Sequence, DNA/genetics, DNA Primers/genetics, Female, Forensic Genetics, Gene Frequency, Genetics, Population, Germany, Humans, Male, Microsatellite Repeats, Mutation",

author = "Dorit Becker and Klaus Bender and Jeanett Edelmann and Frank G{\"o}tz and Lotte Henke and Sandra Hering and Carsten Hohoff and Karolin Hoppe and Michael Klintschar and Matthias Muche and Burkhard Rolf and Reinhard Szibor and Volker Weirich and Martin Jung and Werner Brabetz",

year = "2007",

month = dec,

doi = "10.1016/j.fsigen.2007.04.001",

language = "English",

volume = "1",

pages = "232--7",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

publisher = "Elsevier Ireland Ltd",

number = "3-4",

}

Becker, D, Bender, K, Edelmann, J, Götz, F, Henke, L, Hering, S, Hohoff, C, Hoppe, K, Klintschar, M, Muche, M, Rolf, B, Szibor, R, Weirich, V, Jung, M & Brabetz, W 2007, 'New alleles and mutational events at 14 STR loci from different German populations', Forensic Science International: Genetics, vol. 1, no. 3-4, pp. 232-7. https://doi.org/10.1016/j.fsigen.2007.04.001

TY - JOUR

T1 - New alleles and mutational events at 14 STR loci from different German populations

AU - Becker, Dorit

AU - Bender, Klaus

AU - Edelmann, Jeanett

AU - Götz, Frank

AU - Henke, Lotte

AU - Hering, Sandra

AU - Hohoff, Carsten

AU - Hoppe, Karolin

AU - Klintschar, Michael

AU - Muche, Matthias

AU - Rolf, Burkhard

AU - Szibor, Reinhard

AU - Weirich, Volker

AU - Jung, Martin

AU - Brabetz, Werner

PY - 2007/12

Y1 - 2007/12

N2 - The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.

AB - The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.

KW - Alleles

KW - Base Sequence

KW - DNA/genetics

KW - DNA Primers/genetics

KW - Female

KW - Forensic Genetics

KW - Gene Frequency

KW - Genetics, Population

KW - Germany

KW - Humans

KW - Male

KW - Microsatellite Repeats

KW - Mutation

U2 - 10.1016/j.fsigen.2007.04.001

DO - 10.1016/j.fsigen.2007.04.001

M3 - Article

C2 - 19083767

SN - 1872-4973

VL - 1

SP - 232

EP - 237

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

IS - 3-4

ER -

New alleles and mutational events at 14 STR loci from different German populations

Abstract

Research Field

Keywords

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