Proteo-lipobeads for the oriented encapsulation of membrane proteins

Pinar Frank; Bernhard Siebenhofer; Theresa Hanzer; Andreas Geiss; Florian Schadauer; Ciril Rozman; Bill Durham; Leslie Loew; Bernd Ludwig; Oliver-M.H. Richter; Christoph Nowak; Renate L. C. Naumann

doi:10.1039/c4sm02646b

Proteo-lipobeads for the oriented encapsulation of membrane proteins

Pinar Frank, Bernhard Siebenhofer, Theresa Hanzer, Andreas Geiss, Florian Schadauer, Ciril Rozman, Bill Durham, Leslie Loew, Bernd Ludwig, Oliver-M.H. Richter, Christoph Nowak, Renate L. C. Naumann

Research output: Contribution to journal › Article › peer-review

Abstract

As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example´using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

Original language	English
Number of pages	3
Journal	Soft Matter
DOIs	https://doi.org/10.1039/c4sm02646b
Publication status	Published - 2 Mar 2015

Research Field

Biosensor Technologies

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title = "Proteo-lipobeads for the oriented encapsulation of membrane proteins",

abstract = "As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example´using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.",

author = "Pinar Frank and Bernhard Siebenhofer and Theresa Hanzer and Andreas Geiss and Florian Schadauer and Ciril Rozman and Bill Durham and Leslie Loew and Bernd Ludwig and Oliver-M.H. Richter and Christoph Nowak and Naumann, {Renate L. C.}",

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doi = "10.1039/c4sm02646b",

language = "English",

journal = "Soft Matter",

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T1 - Proteo-lipobeads for the oriented encapsulation of membrane proteins

AU - Frank, Pinar

AU - Siebenhofer, Bernhard

AU - Hanzer, Theresa

AU - Geiss, Andreas

AU - Schadauer, Florian

AU - Rozman, Ciril

AU - Durham, Bill

AU - Loew, Leslie

AU - Ludwig, Bernd

AU - Richter, Oliver-M.H.

AU - Nowak, Christoph

AU - Naumann, Renate L. C.

PY - 2015/3/2

Y1 - 2015/3/2

N2 - As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example´using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

AB - As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example´using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

U2 - 10.1039/c4sm02646b

DO - 10.1039/c4sm02646b

M3 - Article

SN - 1744-683X

JO - Soft Matter

JF - Soft Matter

ER -

Proteo-lipobeads for the oriented encapsulation of membrane proteins

Abstract

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