Abstract
Platinum nanoparticles (Pt-NPs) were generated by pulsed laser ablation in liquid and
proved to be stable in aqueous dispersion for at least six months when stabilized by small amount of
sodium citrate. Their nanozyme activity was tested photometrically with tetramethylbenzidine (TMB) in
the absence and presence of hydrogen peroxide (H2O2) at 22°C, which lead to specific activities (SA)
of 1.9U/mg Pt-NPs and 375U/mg Pt-NPs and limits of detection (LOD) of 1.86pmol/L and
0.026pmol/L, respectively. In comparison, a SA of 289U/mg and a LOD of 36pmol/L were determined
for a recombinant horseradish peroxidase in presence of H2O2 at 22°C. In particular, the lower detection
limit of Pt-NPs by a factor of 1384 compared to the recombinant horseradish peroxidase indicates
their great potential for future bioassays.
proved to be stable in aqueous dispersion for at least six months when stabilized by small amount of
sodium citrate. Their nanozyme activity was tested photometrically with tetramethylbenzidine (TMB) in
the absence and presence of hydrogen peroxide (H2O2) at 22°C, which lead to specific activities (SA)
of 1.9U/mg Pt-NPs and 375U/mg Pt-NPs and limits of detection (LOD) of 1.86pmol/L and
0.026pmol/L, respectively. In comparison, a SA of 289U/mg and a LOD of 36pmol/L were determined
for a recombinant horseradish peroxidase in presence of H2O2 at 22°C. In particular, the lower detection
limit of Pt-NPs by a factor of 1384 compared to the recombinant horseradish peroxidase indicates
their great potential for future bioassays.
| Original language | English |
|---|---|
| Title of host publication | EUROSENSORS XXXVI |
| Chapter | PT6 - Biochemical sensors, Microfluidics, Lab-on-a-Chip and Organ-On-Chip Systems |
| Pages | 425 - 426 |
| Number of pages | 2 |
| ISBN (Electronic) | 978-3-910600-03-4 |
| DOIs | |
| Publication status | Published - 16 Sept 2024 |
Publication series
| Name | Poster |
|---|
Research Field
- Molecular Diagnostics