Abstract
A sensitive method for the quantification of free salbutamol in human urine is described. Sample clean up is performed using SPE an a mixed phase extraction column. Derivatisation is performed with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) and the extract is analysed by GC-MS. The method was found to be suitable for use in the doping field, where a cut-off limit of 1 ILg salbutamol/mL urine is set by the International Olympic Committee (IOC) and approved by the World Anti-Doping Agency (WADA). Above that value a doping violation occurs. In addition, the stability of salbutamol in human urine has been evaluated.
| Original language | English |
|---|---|
| Pages (from-to) | 110-114 |
| Number of pages | 5 |
| Journal | Journal of Separation Science |
| Publication status | Published - 2004 |
Research Field
- Biosensor Technologies
Keywords
- salbutamol
- gas chromatography
- mass spectrometry
- solid phase extraction
- urine
- doping