RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Filip Staniszewski; Christina Schmidleithner; Stefan Schrittwieser; Maximilian Westenthanner; Lidiya Osinkina; Yasmin Gillitschka; Barbara Posch; Joachim Stehr; Johannes Rudolf Peham

RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Filip Staniszewski (Speaker), Christina Schmidleithner, Stefan Schrittwieser, Maximilian Westenthanner, Lidiya Osinkina, Yasmin Gillitschka, Barbara Posch, Joachim Stehr, Johannes Rudolf Peham (Speaker)

Molecular Diagnostics

HP Health Solutions

Research output: Poster presentation without proceedings

Abstract

Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially,
regarding development time and user friendliness. Point-of-care devices, that require little user interaction or
laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that
enables controlled volume deposition independent of applied sample volume and only requires the use of a
disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which
can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic
capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with
further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

Original language	English
Publication status	Published - 29 Sept 2023
Event	Micro and Nanoengineering 2023 - Berlin, Berlin, Germany Duration: 26 Sept 2023 → 28 Sept 2023 https://mne-2023.org/

Conference

Conference	Micro and Nanoengineering 2023
Abbreviated title	MNE
Country/Territory	Germany
City	Berlin
Period	26/09/23 → 28/09/23
Internet address	https://mne-2023.org/

Research Field

Biosensor Technologies

Keywords

microfluidics
SARS-CoV-2
self-filling
LAMP

Cite this

@conference{28059592ec344e58b5fe820731cc5c4c,

title = "RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION",

abstract = "Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments. ",

keywords = "microfluidics, SARS-CoV-2, self-filling, LAMP",

author = "Filip Staniszewski and Christina Schmidleithner and Stefan Schrittwieser and Maximilian Westenthanner and Lidiya Osinkina and Yasmin Gillitschka and Barbara Posch and Joachim Stehr and Peham, {Johannes Rudolf}",

year = "2023",

month = sep,

day = "29",

language = "English",

note = "Micro and Nanoengineering 2023, MNE ; Conference date: 26-09-2023 Through 28-09-2023",

url = "https://mne-2023.org/",

}

TY - CONF

T1 - RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

AU - Schmidleithner, Christina

AU - Schrittwieser, Stefan

AU - Westenthanner, Maximilian

AU - Osinkina, Lidiya

AU - Gillitschka, Yasmin

AU - Posch, Barbara

AU - Stehr, Joachim

A2 - Staniszewski, Filip

A2 - Peham, Johannes Rudolf

PY - 2023/9/29

Y1 - 2023/9/29

N2 - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

AB - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

KW - microfluidics

KW - SARS-CoV-2

KW - self-filling

KW - LAMP

M3 - Poster presentation without proceedings

T2 - Micro and Nanoengineering 2023

Y2 - 26 September 2023 through 28 September 2023

ER -

RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Abstract

Conference

Research Field

Keywords

Fingerprint

Cite this