RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Filip Staniszewski; Christina Schmidleithner; Stefan Schrittwieser; Maximilian Westenthanner; Lidiya Osinkina; Yasmin Gillitschka; Barbara Posch; Joachim Stehr; Johannes Rudolf Peham

RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Filip Staniszewski (Speaker)
, Christina Schmidleithner
, Stefan Schrittwieser
, Maximilian Westenthanner
, Lidiya Osinkina
, Yasmin Gillitschka
, Barbara Posch
, Joachim Stehr
, Johannes Rudolf Peham (Speaker)

Molecular Diagnostics

HP Health Solutions

Research output: Poster presentation without proceedings

Abstract

Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially,
regarding development time and user friendliness. Point-of-care devices, that require little user interaction or
laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that
enables controlled volume deposition independent of applied sample volume and only requires the use of a
disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which
can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic
capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with
further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

Original language	English
Publication status	Published - 29 Sept 2023
Event	Micro and Nanoengineering 2023 - Berlin, Berlin, Germany Duration: 26 Sept 2023 → 28 Sept 2023 https://mne-2023.org/

Conference

Conference	Micro and Nanoengineering 2023
Abbreviated title	MNE
Country/Territory	Germany
City	Berlin
Period	26/09/23 → 28/09/23
Internet address	https://mne-2023.org/

Research Field

Biosensor Technologies

Keywords

microfluidics
SARS-CoV-2
self-filling
LAMP

Cite this

@conference{28059592ec344e58b5fe820731cc5c4c,

title = "RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION",

abstract = "Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60\% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments. ",

keywords = "microfluidics, SARS-CoV-2, self-filling, LAMP",

author = "Filip Staniszewski and Christina Schmidleithner and Stefan Schrittwieser and Maximilian Westenthanner and Lidiya Osinkina and Yasmin Gillitschka and Barbara Posch and Joachim Stehr and Peham, \{Johannes Rudolf\}",

year = "2023",

month = sep,

day = "29",

language = "English",

note = "Micro and Nanoengineering 2023, MNE ; Conference date: 26-09-2023 Through 28-09-2023",

url = "https://mne-2023.org/",

}

TY - CONF

T1 - RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

AU - Schmidleithner, Christina

AU - Schrittwieser, Stefan

AU - Westenthanner, Maximilian

AU - Osinkina, Lidiya

AU - Gillitschka, Yasmin

AU - Posch, Barbara

AU - Stehr, Joachim

A2 - Staniszewski, Filip

A2 - Peham, Johannes Rudolf

PY - 2023/9/29

Y1 - 2023/9/29

N2 - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

AB - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

KW - microfluidics

KW - SARS-CoV-2

KW - self-filling

KW - LAMP

M3 - Poster presentation without proceedings

T2 - Micro and Nanoengineering 2023

Y2 - 26 September 2023 through 28 September 2023

ER -

RAPID PROTOTYPING OF SELF-FILLING MICROFLUIDIC CHIPS FOR SARS-COV-2 RNA DETECTION BY ISOTHERMAL AMPLIFICATION

Abstract

Conference

Research Field

Keywords

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Cite this