Rolling circle amplification (RCA) for enhancement of the sensor response utilizing surface plasmon resonance and surface plasmon-enhanced fluorescence spectroscopy is reported. In order to maximize the efficiency of RCA, dedicated biointerface is developed for specific capture of target analyte and to guide the generated long oligonucleotide chains at the sensor surface within the confined probing surface plasmon field. The enhancement of the limit of detection by two orders of magnitude to 260 fM concentration is achieved for molecular ensembles and possible counting of individual captured molecules enables reaching limit of detection at low fM concentrations.
|SPIE Photonics Europe 2022
|3/04/22 → 7/04/22