Rolling Circle Amplifiaction Tailored for Plasmonic Biosensors

Katharina Schmidt, Bernadette Lechner, Simone Hageneder, Stefan Fossati, Yasaman Ahmadi, Maria Minunni, Erik Reimhult, Ivan Barisic, Jakub Dostalek (Speaker)

Research output: Chapter in Book or Conference ProceedingsConference Proceedings with Oral Presentationpeer-review


Rolling circle amplification (RCA) for enhancement of the sensor response utilizing surface plasmon resonance and surface plasmon-enhanced fluorescence spectroscopy is reported. In order to maximize the efficiency of RCA, dedicated biointerface is developed for specific capture of target analyte and to guide the generated long oligonucleotide chains at the sensor surface within the confined probing surface plasmon field. The enhancement of the limit of detection by two orders of magnitude to 260 fM concentration is achieved for molecular ensembles and possible counting of individual captured molecules enables reaching limit of detection at low fM concentrations.
Original languageEnglish
Title of host publicationSPIE Biophotonics in Point-of-Care II
Number of pages1
Publication statusPublished - 2022
EventSPIE Photonics Europe 2022 -
Duration: 3 Apr 20227 Apr 2022


ConferenceSPIE Photonics Europe 2022

Research Field

  • Molecular Diagnostics


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