SELF FILLING MICROLFUIDIC CHIP PRODUCED BY RAPID INJECTION MOLDING FOR ISOTHERMAL SARS-COV-2 DETECTION

Filip Staniszewski; Christina Schmidleithner; Lidiya  Osinkina; Stefan Schrittwieser; Yasmin Gillitschka; Barbara Posch; Maximilian Westenthanner; Joachim Stehr; Johannes Rudolf Peham

SELF FILLING MICROLFUIDIC CHIP PRODUCED BY RAPID INJECTION MOLDING FOR ISOTHERMAL SARS-COV-2 DETECTION

Filip Staniszewski (Speaker), Christina Schmidleithner, Lidiya Osinkina, Stefan Schrittwieser, Yasmin Gillitschka, Barbara Posch, Maximilian Westenthanner, Joachim Stehr, Johannes Rudolf Peham

Molecular Diagnostics

HP Health Solutions

Research output: Poster presentation without proceedings

Abstract

Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially,
regarding development time and user friendliness. Point-of-care devices, that require little user interaction or
laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that
enables controlled volume deposition independent of applied sample volume and only requires the use of a
disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which
can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic
capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with
further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

Original language	English
Publication status	Published - 15 Sept 2023
Event	BioMedEng 23 - Swansea, Swansea, United Kingdom Duration: 13 Sept 2023 → 13 Oct 2023 https://biomedeng.org/biomedeng23/

Conference

Conference	BioMedEng 23
Country/Territory	United Kingdom
City	Swansea
Period	13/09/23 → 13/10/23
Internet address	https://biomedeng.org/biomedeng23/

Research Field

Biosensor Technologies

Keywords

Microfluidics
self-filling
LAMP
SARS-CoV-2

Cite this

@conference{535cb1fc0d71418094d60781f285d9fd,

title = "SELF FILLING MICROLFUIDIC CHIP PRODUCED BY RAPID INJECTION MOLDING FOR ISOTHERMAL SARS-COV-2 DETECTION",

abstract = "Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments. ",

keywords = "Microfluidics, self-filling, LAMP, SARS-CoV-2",

author = "Filip Staniszewski and Christina Schmidleithner and Lidiya Osinkina and Stefan Schrittwieser and Yasmin Gillitschka and Barbara Posch and Maximilian Westenthanner and Joachim Stehr and Peham, {Johannes Rudolf}",

year = "2023",

month = sep,

day = "15",

language = "English",

note = "BioMedEng 23 ; Conference date: 13-09-2023 Through 13-10-2023",

url = "https://biomedeng.org/biomedeng23/",

}

TY - CONF

T1 - SELF FILLING MICROLFUIDIC CHIP PRODUCED BY RAPID INJECTION MOLDING FOR ISOTHERMAL SARS-COV-2 DETECTION

AU - Schmidleithner, Christina

AU - Osinkina, Lidiya

AU - Schrittwieser, Stefan

AU - Gillitschka, Yasmin

AU - Posch, Barbara

AU - Westenthanner, Maximilian

AU - Stehr, Joachim

AU - Peham, Johannes Rudolf

A2 - Staniszewski, Filip

PY - 2023/9/15

Y1 - 2023/9/15

N2 - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

AB - Since the Sars-CoV-2 pandemic the public interest in diagnostic testing has dramatically increased, especially, regarding development time and user friendliness. Point-of-care devices, that require little user interaction or laboratory equipment, are desired. Here we propose a microfluidic chip based on capillary retention valves, that enables controlled volume deposition independent of applied sample volume and only requires the use of a disposable transfer pipette. For rapid deployment, a pipeline for accelerated prototyping was established, which can save up to 60% in costs and several weeks in time compared to conventional prototyping. The diagnostic capability of this microfluidic chip was achieved by incorporating a LAMP assay for the detection of viral SarsCoV-2 RNA at a limit of detection of 10 copies per µL. In future, this microfluidic design can be combined with further assays to provide rapid and cost-effective pathogen detection without any further readout instruments.

KW - Microfluidics

KW - self-filling

KW - LAMP

KW - SARS-CoV-2

M3 - Poster presentation without proceedings

T2 - BioMedEng 23

Y2 - 13 September 2023 through 13 October 2023

ER -

SELF FILLING MICROLFUIDIC CHIP PRODUCED BY RAPID INJECTION MOLDING FOR ISOTHERMAL SARS-COV-2 DETECTION

Abstract

Conference

Research Field

Keywords

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