Abstract
Immune mediated inflammatory diseases (IMIDs) are a heterogeneous group of conditions featured by a dysregulated immune response leading to destructive chronic inflammation. The immune dysregulation can affect various organ systems (e.g. gut, skin) resulting in significant morbidity, reduced quality of life and premature death. As there are no reliable disease progression and therapy response biomarkers currently available, ImmUniverse, a large, EU-funded project, is aiming to develop such biomarkers in two IMIDs, namely atopic dermatitis (AD) and ulcerative colitis (UC) not least by means of small RNA/microRNA profiling in blood-derived extracellular vesicles (EVs).
Materials & Methods
small RNA sequencing was performed on total RNA which has been isolated from plasma- or serum-derived EVs. Analysed samples comprised atopic dermatitis patients, healthy controls as well as blood samples derived from ulcerative colitis patients before or after treatment with biologicals. In addition, mouse plasma samples were investigated for the effect of Low-Intensity Pulsed Ultrasound (LIPUS) as a novel and safe approach to enhance the mucosal release of EVs in experimental models of UC.
Results
We identified a variety of potential EV-derived small RNA biomarkers in AD and UC retrospective patients and could nicely show the impact of LIPUS on EV release into mouse plasma.
Discussion
EV-derived small RNA biomarkers are a novel and promising source of diagnostic biomarkers in IMIDs but by no means are limited to this type of conditions as we also demonstrate by exemplarily showcasing some within AIT identified epigenetic biomarkers for coronary artery disease.
Materials & Methods
small RNA sequencing was performed on total RNA which has been isolated from plasma- or serum-derived EVs. Analysed samples comprised atopic dermatitis patients, healthy controls as well as blood samples derived from ulcerative colitis patients before or after treatment with biologicals. In addition, mouse plasma samples were investigated for the effect of Low-Intensity Pulsed Ultrasound (LIPUS) as a novel and safe approach to enhance the mucosal release of EVs in experimental models of UC.
Results
We identified a variety of potential EV-derived small RNA biomarkers in AD and UC retrospective patients and could nicely show the impact of LIPUS on EV release into mouse plasma.
Discussion
EV-derived small RNA biomarkers are a novel and promising source of diagnostic biomarkers in IMIDs but by no means are limited to this type of conditions as we also demonstrate by exemplarily showcasing some within AIT identified epigenetic biomarkers for coronary artery disease.
| Original language | English |
|---|---|
| Title of host publication | Small New World 2.0 program and abstracts |
| Publication status | Published - 5 Sept 2023 |
| Event | Small New World 2.0 - Graz, Austria Duration: 4 Sept 2023 → 5 Sept 2023 https://asev.at/documents/SmallNewWorld2.0%20-%202023%20-%20Abstract%20book.pdf |
Conference
| Conference | Small New World 2.0 |
|---|---|
| Country/Territory | Austria |
| City | Graz |
| Period | 4/09/23 → 5/09/23 |
| Internet address |
Research Field
- Molecular Diagnostics