Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Naoto Asai; Gizem Aktug; Andres de los Santos; Thomás Riedel; Jakub Dostalek

doi:10.1117/12.2669182

Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Naoto Asai (Speaker), Gizem Aktug, Andres de los Santos, Thomás Riedel, Jakub Dostalek

Research output: Chapter in Book or Conference Proceedings › Conference Proceedings with Oral Presentation › peer-review

Abstract

To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.

Original language	English
Title of host publication	PROCEEDINGS VOLUME 12572
Editors	Francesco Baldini, Jiri Homola, Robert A. Lieberman
Volume	12572
DOIs	https://doi.org/10.1117/12.2669182
Publication status	Published - 31 May 2023
Event	SPIE Optics + Optoelectronics: Optical Sensors 2023 - Prague, Czech Republic Duration: 24 Apr 2023 → 27 Apr 2023 Conference number: 12572 https://spie.org/conferences-and-exhibitions/optics-and-optoelectronics

Publication series

Name	Optical Sensors 2023

Conference

Conference	SPIE Optics + Optoelectronics
Country/Territory	Czech Republic
City	Prague
Period	24/04/23 → 27/04/23
Internet address	https://spie.org/conferences-and-exhibitions/optics-and-optoelectronics

Research Field

Biosensor Technologies

Access to Document

10.1117/12.2669182

Cite this

@inproceedings{5c962b56cde249aaa40d53c1c90db40c,

title = "Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)",

abstract = "To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.",

author = "Naoto Asai and Gizem Aktug and {de los Santos}, Andres and Thom{\'a}s Riedel and Jakub Dostalek",

year = "2023",

month = may,

day = "31",

doi = "10.1117/12.2669182",

language = "English",

volume = "12572",

series = "Optical Sensors 2023",

editor = "Francesco Baldini and Jiri Homola and Lieberman, {Robert A.}",

booktitle = "PROCEEDINGS VOLUME 12572",

note = "SPIE Optics + Optoelectronics : Optical Sensors 2023 ; Conference date: 24-04-2023 Through 27-04-2023",

url = "https://spie.org/conferences-and-exhibitions/optics-and-optoelectronics",

}

Asai, N, Aktug, G, de los Santos, A, Riedel, T & Dostalek, J 2023, Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation). in F Baldini, J Homola & RA Lieberman (eds), PROCEEDINGS VOLUME 12572. vol. 12572, Optical Sensors 2023, SPIE Optics + Optoelectronics, Prague, Czech Republic, 24/04/23. https://doi.org/10.1117/12.2669182

Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation). / Asai, Naoto (Speaker); Aktug, Gizem; de los Santos, Andres et al.
PROCEEDINGS VOLUME 12572. ed. / Francesco Baldini; Jiri Homola; Robert A. Lieberman. Vol. 12572 2023. (Optical Sensors 2023).

Research output: Chapter in Book or Conference Proceedings › Conference Proceedings with Oral Presentation › peer-review

TY - GEN

T1 - Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

AU - Aktug, Gizem

AU - de los Santos, Andres

AU - Riedel, Thomás

AU - Dostalek, Jakub

A2 - Asai, Naoto

A2 - Baldini, Francesco

A2 - Homola, Jiri

A2 - Lieberman, Robert A.

N1 - Conference code: 12572

PY - 2023/5/31

Y1 - 2023/5/31

N2 - To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.

AB - To achieve single-molecule detection based on enzyme-free and isothermal amplification techniques, we have developed a strategy employing coupled catalytic hairpin assembly reactions and investigated several parameters influencing its yield. We employed avidin-biotin assay using biotin-modified trigger DNA and surface plasmon resonance to excite fluorescence-labeled hairpin DNA captured a surface. Our investigation of association and dissociation of fluorescence-labeled hairpin DNA allows to determine the reaction yield and provide guide to design the involved oligonucleotides.

UR - https://www.mendeley.com/catalogue/026b15a9-d68d-3554-9a68-3878f36c5070/

U2 - 10.1117/12.2669182

DO - 10.1117/12.2669182

M3 - Conference Proceedings with Oral Presentation

VL - 12572

T3 - Optical Sensors 2023

BT - PROCEEDINGS VOLUME 12572

T2 - SPIE Optics + Optoelectronics

Y2 - 24 April 2023 through 27 April 2023

ER -

Surface plasmon coupled fluorescence biosensor with enzyme-free catalytic hairpin assembly amplification (Conference Presentation)

Abstract

Publication series

Conference

Research Field

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