UV-Laser Interference Lithography for Local Functionalization of Plasmonic Nanostructures with Responsive Hydrogel

Nestor Gisbert Quilis, Simone Hageneder, Stefan Fossati, Simone K. Auer, Priyamvada Venugopalan, Anil Bozdogan, Christian Petri, Alberto Moreno-Cencerrado, Jose Luis Toca-Herrera, Ulrich Jonas, Jakub Dostalek

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A novel approach to local functionalization of plasmonic hotspots at gold nanoparticles with biofunctional moieties is reported. It relies on photocrosslinking and attachment of a responsive hydrogel binding matrix by the use of a UV interference field. A thermoresponsive poly(N-isopropylacrylamide)-based (pNIPAAm) hydrogel with photocrosslinkable benzophenone groups and carboxylic groups for its postmodification was employed. UV-laser interference lithography with a phase mask configuration allowed for the generation of a high-contrast interference field that was used for the recording of periodic arrays of pNIPAAm-based hydrogel features with the size as small as 170 nm. These hydrogel arrays were overlaid and attached on the top of periodic arrays of gold nanoparticles, exhibiting a diameter of 130 nm and employed as a three-dimensional binding matrix in a plasmonic biosensor. Such a hybrid material was postmodified with ligand biomolecules and utilized for plasmon-enhanced fluorescence readout of an immunoassay. Additional enhancement of the fluorescence sensor signal by the collapse of the responsive hydrogel binding matrix that compacts the target analyte at the plasmonic hotspot is demonstrated.
    Original languageEnglish
    Pages (from-to)3297-3305
    Number of pages9
    JournalJournal of Physical Chemistry C
    Volume124
    Issue number5
    DOIs
    Publication statusPublished - 2020

    Research Field

    • Biosensor Technologies

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